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After Alcmaeon’s findings, many scientists, philosophers, and physicians from around the world continued to contribute to the understanding of neuroanatomy, notably: Galen, Herophilus, Rhazes and Erasistratus.

Herophilus and Erasistratus of Alexandria were perhaps the most influential Greek neuroscientists with their studies involving dissecting the brains.

A mid-sagittal plane divides the body and brain into left and right halves; sagittal sections in general are parallel to this median plane, moving along the medial-lateral dimension(see the image above).

The term sagittal refers etymologically to the median suture between the right and left parietal bones of the cranium, known classically as sagittal suture, because it looks roughly like an arrow by its confluence with other sutures (sagitta; arrow in Latin).

Histochemistry uses knowledge about biochemical reaction properties of the chemical constituents of the brain (including notably enzymes) to apply selective methods of reaction to visualize where they occur in the brain and any functional or pathological changes.

Immunoreacted transcription factor proteins reveal genomic readout in terms of translated protein.

This immensely increases the capacity of researchers to distinguish between different cell types (such as neurons and glia) in various regions of the nervous system.

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